MetaMorph 7.6 is the next generation of world-class image acquisition and analysis software from Molecular Devices.

Combining the most flexible and powerful tools for image acquisition, processing, and analysis, MetaMorph imaging systems offer a complete solution for even the most demanding live-cell imaging needs.

MetaMorph 7.6 now includes two new applications; 3D Track Objects and the Micronuclei Application Module.

Key Features:

• Acquisition & Device Control MetaMorph device control makes acquisition fast and easy, allowing the integration of automated microscopes and a variety other hardware devices into a single, intuitive acquisition interface.
• Image Display & Processing Image display and processing tools are crucial for performing accurate image analysis, and MetaMorph offers a complete toolbox of advanced features in this area, from background subtraction and shading correction, to morphology filters and an interactive 4D Viewer.
• Image Analysis MetaMorph offers analysis tools to handle everything from simple intensity logging to advanced morphometry analysis, colocalization, FRET, 3D measurements, and more. Optional Application Modules perform assay-specific analysis, such as counting nuclei or assessing cell cycle phases, in a simple wizard-like interface.
• Customization Powerful and easy-to-use journal (macro) functions in MetaMorph enable users to further automate acquisition, processing, and common analysis routines, while custom toolbars and menus provide users quick access to commonly used menu options, taskbars, and journals.

Fluorescence ratio imaging is the monitoring of live cells in which a fluorescent indicator of intracellular ions is introduced. Indicator dyes have been designed to shift their fluorescence excitation or emission spectrum when binding with specific ions. Images are obtained at two different wavelengths, typically matching the absorption bands at the high and low binding conditions.

By ratioing the intensities in the images, it is possible to construct a map showing the local ion concentrations throughout the field of view. Since the monitoring process is nondestructive, image acquisition can be repeated frequently to trace and monitor the time course of cellular responses. The MetaFluor® Imaging System is designed for dual-wavelength intracellular ion measurements. The system provides simultaneous display of the raw data, ratio image, graphs of intensities, ratios and ion concentrations, and a non-ratiometric image such as a brightfield or phase-contrast image. Two different ratiometric indicators can be imaged and measured simultaneously.

Custom Configuration
Toolbars, menus, wizards and dialog boxes help move you through the image processing steps quickly. Features such as multiple image windows, flexible device control, synchronization and timing, and journals allow for automated image acquisition and analysis unlike any other system. With MetaFluor, you customize the set-up once, then let the experiment run by itself. You are able to collect a large amount of data online and process it with either MetaFluor or an analysis-only copy of the software.

The MetaVue™ Imaging System is the cost-effective solution for basic imaging applications such as digital photography, multi-wavelength fluorescence, image processing and image analysis. MetaVue is a simple, easy-to-use system for acquiring and processing images, performing graphics functions, and archiving and retrieving images.

For multi-mode microscopy, MetaVue is able to acquire multiple fluorescence wavelengths combined with a transmitted light image. Images can be stacked into one file and saved as a Stack file format, then processed or measured all at once in one easy step.

With the MetaVue system's modular architecture, expanding the software is simple and allows the system to grow as your imaging needs change. Compatibility with MetaMorph® and MetaFluor® offers a well-defined upgrade path.